《植物生理学报》 2017, 53(2): 257-263

高温胁迫下根施褪黑素对葡萄叶片叶绿素荧光特性的影响

卞凤娥, 孙永江, 牛彦杰, 杜远鹏, 翟衡^*

山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室, 山东泰安271018

通信作者：翟；E-mail: zhaih@sdau.edu.cn

摘　要：

本文以盆栽‘威代尔’葡萄为试验材料, 通过测定叶绿素荧光快速诱导动力学曲线、叶绿素荧光淬灭并结合气体交换, 分析了高温胁迫下根施褪黑素(melatonin, MT)对葡萄叶片叶绿素荧光特性的影响。结果表明, 高温胁迫下, 与对照(CK)相比, 葡萄叶片净光合速率(P_n)及叶片最大光化学效率(F_v/F_m)均显著下降, OJIP曲线形状发生明显改变, 非光化学淬灭(NPQ)显著升高; 而根施褪黑素显著缓解了叶片P_n和F_v/F_m的下降, 明显降低了高温胁迫下OJIP曲线中K点和J点上升的幅度, 增加了叶片对光能的利用效率。上述结果表明, 高温胁迫导致葡萄叶片光系统II (PSII)发生较严重的伤害, 根施褪黑素能够缓解PSII活性和光合速率的下降程度, 改善高温胁迫下葡萄叶片的能量分配。

关键词：高温胁迫; 褪黑素; 葡萄; 光合作用; PSII活性; 叶绿素荧光

收稿：2016-08-25   修定：2017-01-04

资助：国家现代葡萄产业技术体系建设专项(CARS-30)和长江学者和创新团队发展计划(IRT15R42)。

Effect of root-applied melatonin on photosystem II in grape leaves under heat stress

BIAN Feng-E, SUN Yong-Jiang, NIU Yan-Jie, DU Yuan-Peng, ZHAI Heng^*

College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai’an, Shandong 271018, China

Corresponding author: ZHAI Heng; E-mail: zhaih@sdau.edu.cn

Abstract:

The potted ‘Vidal Blanc’ grapevines were used to investigate the effect of root-applied melatonin on photosynthetic characteristics of grape leaves under high temperature. OJIP fluorescence transient, chlorophyll fluorescence quenching and gas exchange parameters were determined. The results showed that P_n (photosynthetic rate) and F_v/F_m (the maximum photochemical efficiency of PSII) were significantly decreased, the shape of the OJIP curve was clearly changed, and NPQ (the non-photochemical quenching) was significantly increased under high temperature compared to the CK conditions. In contrast, the root-applied melatonin alleviated the decreases in the parameters of P_n and F_v/F_m, declined the increasing amplitude of the K and J points and enhanced photosynthesis rate clearly under high temperature. Therefore, high temperature stress resulted in a severe damage of PSII, root-applied melatonin alleviated the decreases in photochemistry activity of PSII and photosynthesis rate, improved the energy distribution of grape leaf.

Key words: heat stress; melatonin; grape; photosynthesis; PSII photochemistry; chlorophyll fluorescence